Investigating Filovirus Expression in Monodelphis domestica

Daniel Stuff, Oberlin College

Location

Science Center, A154

Document Type

Presentation

Start Date

4-24-2015 4:00 PM

End Date

4-24-2015 5:30 PM

Abstract

In this study, we explored the expression of a filovirus nucleoprotein (NP) gene and a filovirus RNA dependent RNA polymerase (L) gene in Monodelphis domestica. While we know that these filovirus genes are maintained within M. domestica’s genome, we do not know if they are expressed. Using primers specific to the NP and L gene, we extracted mRNA from various M. domestica tissues and attempted to see if any and how much amplification would occur using the primers for polymerase chain reaction.

Notes

Session 3, Panel 15 - Biology Honors
Moderator: Marta Laskowski, Professor of Biology

Major

Biology

Advisor(s)

Yolanda Cruz, Biology

Project Mentor(s)

Yolanda Cruz, Biology

April 2015

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Apr 24th, 4:00 PM Apr 24th, 5:30 PM

Investigating Filovirus Expression in Monodelphis domestica

Science Center, A154

In this study, we explored the expression of a filovirus nucleoprotein (NP) gene and a filovirus RNA dependent RNA polymerase (L) gene in Monodelphis domestica. While we know that these filovirus genes are maintained within M. domestica’s genome, we do not know if they are expressed. Using primers specific to the NP and L gene, we extracted mRNA from various M. domestica tissues and attempted to see if any and how much amplification would occur using the primers for polymerase chain reaction.