Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125

Tae Kyu (Brian) Uhm, Oberlin College

Location

Science Center, A155

Document Type

Presentation

Start Date

4-25-2014 1:30 PM

End Date

4-25-2014 2:30 PM

Abstract

Ovarian cancer biomarker CA125 is widely used to diagnose and monitor ovarian cancer. In order to develop novel diagnostic and therapeutic tools, nucleic acid aptamers with high affinity to CA125 were selected using One-Pot based systematic evolution of ligands by exponential enrichment (One-Pot SELEX). After several rounds of One-Pot SELEX, selected aptamers were sequenced using high-throughput sequencing and analyzed. Dissociation constants of selected aptamers were determined using affinity probe capillary electrophoresis and fluorescence anisotropy. This work is supported by the National Cancer Institute.

Notes

Session I, Panel 2 - Meiosis, Memory, Mutation: Studies in Reproduction, Menopause, and Ovarian Cancer
Moderator: Tracie Paine, Associate Professor of Neuroscience

Major

Chemistry and Biochemistry

Advisor(s)

Robert Thompson, Chemistry and Biochemistry

Project Mentor(s)

Rebecca Whelan, Chemistry and Biochemistry

April 2014

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Apr 25th, 1:30 PM Apr 25th, 2:30 PM

Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125

Science Center, A155

Ovarian cancer biomarker CA125 is widely used to diagnose and monitor ovarian cancer. In order to develop novel diagnostic and therapeutic tools, nucleic acid aptamers with high affinity to CA125 were selected using One-Pot based systematic evolution of ligands by exponential enrichment (One-Pot SELEX). After several rounds of One-Pot SELEX, selected aptamers were sequenced using high-throughput sequencing and analyzed. Dissociation constants of selected aptamers were determined using affinity probe capillary electrophoresis and fluorescence anisotropy. This work is supported by the National Cancer Institute.