The Phylogenetic Reach of Hybridization-Based Enrichment

Rebecca Mostow, Oberlin College

Location

Science Center, A154

Document Type

Presentation

Start Date

4-26-2013 4:00 PM

End Date

4-26-2013 5:00 PM

Abstract

The goal of this project is to evaluate the efficacy of hybridization-based DNA enrichment over a wide phylogenetic range. Hybridization-based enrichment uses RNA probes to identify and extract multiple regions of interest from sampled DNA. In the case of this study, the regions of interest are the nuclear genomes of several groups of gypsum endemic plants. Baits are traditionally designed from and for the genome of a single species. I will use baits to enrich the nuclear genomes of species closely and distantly related to the bait source species. I will then use this data to analyze the effect of phylogenetic distance between bait source and target species on the efficacy enrichment.

Notes

Session III, Panel 11: Mapping the Genus: Studies of Flora and Phylogeny
Moderator: Mike Moore, Associate Professor of Biology

Major

Biology

Advisor(s)

Katherine Cullen, Biology

Project Mentor(s)

Michael Moore, Biology

April 2013

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Apr 26th, 4:00 PM Apr 26th, 5:00 PM

The Phylogenetic Reach of Hybridization-Based Enrichment

Science Center, A154

The goal of this project is to evaluate the efficacy of hybridization-based DNA enrichment over a wide phylogenetic range. Hybridization-based enrichment uses RNA probes to identify and extract multiple regions of interest from sampled DNA. In the case of this study, the regions of interest are the nuclear genomes of several groups of gypsum endemic plants. Baits are traditionally designed from and for the genome of a single species. I will use baits to enrich the nuclear genomes of species closely and distantly related to the bait source species. I will then use this data to analyze the effect of phylogenetic distance between bait source and target species on the efficacy enrichment.