Determination of Global Structure from Distance and Orientation Constraints in Biological Solids Using Solid-State NMR Spectroscopy
Abstract
We report the results from a new solid-state NMR experiment, DANTE−REDOR, which can determine global secondary structure in uniformly (13C,15N)-enriched systems by simultaneously measuring distance and orientation constraints. Following a heteronuclear spin-pair selection using a DANTE pulse train, the magnitude and orientation of the internuclear dipole vector, within the chemical shift anisotropy (CSA) frame of the observed nucleus, are determined by tracking the dephasing of individual spinning sidebands under magic angle spinning. The efficacy of the experiment is demonstrated by measuring the imidazole side-chain orientation in U-[13C6,15N3]-l-histidine·HCl·H2O.
Repository Citation
Andreas, Loren B., Loren B. Andreas, and Anil K. Mehta. 2007. "Determination of Global Structure from Distance and Orientation Constraints in Biological Solids Using Solid-State NMR Spectroscopy." Journal Of The American Chemical Society 129(49): 15233-15239.
Publisher
American Chemical Society
Publication Date
1-1-2007
Publication Title
Journal of the American Chemical Society
Department
Chemistry and Biochemistry
Document Type
Article
DOI
https://dx.doi.org/10.1021/ja074789q
Language
English
Format
text
