Event Title
Receptor Tyrosine Kinase Activity in Angiosarcoma
Location
Science Center, Bent Corridor
Start Date
10-2-2015 12:00 PM
End Date
10-2-2015 1:20 PM
Research Program
Summer Undergraduate Research Fellowship (SURF), University of Cincinnati
Poster Number
20
Abstract
Background and Rationale. Angiosarcomas are a rare and lethal form of vascular tumor. While therapeutic irradiation and treatment-related chronic lymphedema are predisposing factors in adults, in a sub-group of patients, including most pediatric cases, angiosarcoma arises without any known environmental exposure. These tumors lack effective treatments due to their unique endothelial cell origin and our limited understanding about their biology. In both human and our murine angiosarcomas there are mutations in protein tyrosine phosphatases that regulate RTKs: PTPRB and Ptpn12 respectively. However little is known about which specific receptors are involved in tumorigenesis. Therefore, we decided to take a candidate approach in an effort to identify these receptors. We chose to focus on RTKs known to play an important role in angiogenesis. Methods. We performed immunoprecipitation using antibodies directed against various receptor tyrosine kinases followed by Western Blotting for the total protein and for tyrosine phosphorylation. Results. Vegfr2 is abundantly expressed in angiosarcoma, however it does not appear to be phosphorylated. We did not detect the presence or phosphorylation of Vegfr1, Vegfr3, and Tie2 on our murine samples. Conclusions: Using a candidate approach, we were not able to determine which RTK is the target of regulation by Ptpn12 in murine angiosarcoma.
Recommended Citation
Thomas, Dana, "Receptor Tyrosine Kinase Activity in Angiosarcoma" (2015). Celebration of Undergraduate Research. 24.
https://digitalcommons.oberlin.edu/cour/2015/posters/24
Major
Neuroscience
Project Mentor(s)
Lionel Chow, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center
Document Type
Poster
Receptor Tyrosine Kinase Activity in Angiosarcoma
Science Center, Bent Corridor
Background and Rationale. Angiosarcomas are a rare and lethal form of vascular tumor. While therapeutic irradiation and treatment-related chronic lymphedema are predisposing factors in adults, in a sub-group of patients, including most pediatric cases, angiosarcoma arises without any known environmental exposure. These tumors lack effective treatments due to their unique endothelial cell origin and our limited understanding about their biology. In both human and our murine angiosarcomas there are mutations in protein tyrosine phosphatases that regulate RTKs: PTPRB and Ptpn12 respectively. However little is known about which specific receptors are involved in tumorigenesis. Therefore, we decided to take a candidate approach in an effort to identify these receptors. We chose to focus on RTKs known to play an important role in angiogenesis. Methods. We performed immunoprecipitation using antibodies directed against various receptor tyrosine kinases followed by Western Blotting for the total protein and for tyrosine phosphorylation. Results. Vegfr2 is abundantly expressed in angiosarcoma, however it does not appear to be phosphorylated. We did not detect the presence or phosphorylation of Vegfr1, Vegfr3, and Tie2 on our murine samples. Conclusions: Using a candidate approach, we were not able to determine which RTK is the target of regulation by Ptpn12 in murine angiosarcoma.
