Mouse blastocyst immunosurgery with commercial antiserum to mouse erythrocytes
Immunosurgery is a useful technique for the isolation of inner cell masses from murine blastocysts. Conventionally, rabbit antisera made ad hoc against murine splenic or fetal cells or fibroblasts have been used as antibody sources. We investigated the feasibility of using commercially available rabbit antiserum to murine erythrocytes (anti-RBC) and compared it with rabbit antiserum generated ad hoc to murine L-cells (anti-L-cell). Our results indicate that anti-RBC is at least as effective as anti-L-cell serum for the immunosurgical isolation of inner cell masses, which became either miniblastocysts (later forming outgrowths) or embryoid bodies (undergoing ectoderm-endodermlike differentiation within 48 h). Because anti-RBC is commercially available, the technical modification described herein increases the accessibility of the immunosurgical protocol for the isolation of murine inner cell masses.
Cruz, Y.P., R.S. Treichel, E. Harsay, and K.D. Chi. 1993. "Mouse blastocyst immunosurgery with commercial antiserum to mouse erythrocytes." In Vitro Cellular and Developmental Biology - Animal 29(8): 671-675.
In Vitro Cellular and Developmental Biology - Animal
Anitbody cytotoxicity, Immunosurgery, Inner cell mass isolation