Event Title

Receptor Tyrosine Kinase Activity in Angiosarcoma

Presenter Information

Dana Thomas, Oberlin College

Location

Science Center, Bent Corridor

Start Date

10-2-2015 12:00 PM

End Date

10-2-2015 1:20 PM

Research Program

Summer Undergraduate Research Fellowship (SURF), University of Cincinnati

Poster Number

20

Abstract

Background and Rationale. Angiosarcomas are a rare and lethal form of vascular tumor. While therapeutic irradiation and treatment-related chronic lymphedema are predisposing factors in adults, in a sub-group of patients, including most pediatric cases, angiosarcoma arises without any known environmental exposure. These tumors lack effective treatments due to their unique endothelial cell origin and our limited understanding about their biology. In both human and our murine angiosarcomas there are mutations in protein tyrosine phosphatases that regulate RTKs: PTPRB and Ptpn12 respectively. However little is known about which specific receptors are involved in tumorigenesis. Therefore, we decided to take a candidate approach in an effort to identify these receptors. We chose to focus on RTKs known to play an important role in angiogenesis. Methods. We performed immunoprecipitation using antibodies directed against various receptor tyrosine kinases followed by Western Blotting for the total protein and for tyrosine phosphorylation. Results. Vegfr2 is abundantly expressed in angiosarcoma, however it does not appear to be phosphorylated. We did not detect the presence or phosphorylation of Vegfr1, Vegfr3, and Tie2 on our murine samples. Conclusions: Using a candidate approach, we were not able to determine which RTK is the target of regulation by Ptpn12 in murine angiosarcoma.

Major

Neuroscience

Project Mentor(s)

Lionel Chow, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center

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Oct 2nd, 12:00 PM Oct 2nd, 1:20 PM

Receptor Tyrosine Kinase Activity in Angiosarcoma

Science Center, Bent Corridor

Background and Rationale. Angiosarcomas are a rare and lethal form of vascular tumor. While therapeutic irradiation and treatment-related chronic lymphedema are predisposing factors in adults, in a sub-group of patients, including most pediatric cases, angiosarcoma arises without any known environmental exposure. These tumors lack effective treatments due to their unique endothelial cell origin and our limited understanding about their biology. In both human and our murine angiosarcomas there are mutations in protein tyrosine phosphatases that regulate RTKs: PTPRB and Ptpn12 respectively. However little is known about which specific receptors are involved in tumorigenesis. Therefore, we decided to take a candidate approach in an effort to identify these receptors. We chose to focus on RTKs known to play an important role in angiogenesis. Methods. We performed immunoprecipitation using antibodies directed against various receptor tyrosine kinases followed by Western Blotting for the total protein and for tyrosine phosphorylation. Results. Vegfr2 is abundantly expressed in angiosarcoma, however it does not appear to be phosphorylated. We did not detect the presence or phosphorylation of Vegfr1, Vegfr3, and Tie2 on our murine samples. Conclusions: Using a candidate approach, we were not able to determine which RTK is the target of regulation by Ptpn12 in murine angiosarcoma.